monoclonal mouse anti abcg2 antibody (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology monoclonal mouse anti abcg2 antibody

Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target <t>ABCG2.</t> Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.

Monoclonal Mouse Anti Abcg2 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 522 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abcg2/ABCG2+Antibody/pmc12915154-2-0-8
Average 96 stars, based on 522 article reviews

monoclonal mouse anti abcg2 antibody - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays"

Article Title: Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays

Journal: STAR Protocols

doi: 10.1016/j.xpro.2026.104361

Figure Legend Snippet: Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target ABCG2. Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.

Techniques Used: Transfection, Plasmid Preparation, Control, Variant Assay, Incubation, Immunofluorescence

Labeling:

Article Title: Gold complex QB1561 suppresses drug-resistant cancer cells by inhibiting TrxR and mitochondrial respiratory function
Article Snippet: .. The primary antibody (1:800) and the secondary antibody labeled with Horseradish Peroxidase (HRP, 1:1,500, Cell Signaling Technology, Dancers, MA, United States) were used to determine the presence of protein, ABCG2 (BXP-21, Santa Cruz Biotechnology, TX, United States) and the internal control GAPDH (Thermo Fisher Scientific, Rockford, IL, United States). ..

Control:

Article Title: Resin Glycosides from Ipomoea funis as Inhibitors of P-Glycoprotein in Multidrug-Resistant Breast Carcinoma Cells.
Article Snippet: P-gp inhibitor agents, reserpine (10 μM, 7551−5G, Merck) and verapamil (10 μM, R1131−1G, Sigma-Aldrich) were used as positive controls.11 .. The reversal of MDR mediated by P-gp and the mechanism of cellular death was evaluated using the following monoclonal antibodies against: caspase-3 (sc-56053, Santa Cruz Biotechnology), ABCB1 (sc55510, Santa Cruz Biotechnology), ABCG2 (sc-58224, Santa Cruz Biotechnology), horseradish peroxidase (HRP)-conjugated β-actin loading control monoclonal antibody (sc-47778, Santa Cruz Biotechnology), and HRP-conjugated antimouse IgG secondary antibody (ab6728, Abcam). .. Cells were collected from 6-well plates (5 × 104 cells/well), which were treated with or without different concentrations of compound 2 for 48 h. Later, cells were treated with lysis buffer containing 292 mM saccharose, 100 mM Tris (pH 7.6), 5 mM magnesium chloride (MgCl2), 0.1% (w/v) sodium dodecyl sulfate (SDS), 1% (w/v) nonyl phenoxypolyethoxylethanol (NP40), 5 mM ethylenediamine tetraacetic acid (EDTA), and protease inhibitor (P9599, Sigma-Aldrich).

Article Title: Resin Glycosides from Ipomoea funis as Inhibitors of P‑Glycoprotein in Multidrug-Resistant Breast Carcinoma Cells
Article Snippet: P-gp inhibitor agents, reserpine (10 μM, 7551–5G, Merck) and verapamil (10 μM, R1131–1G, Sigma-Aldrich) were used as positive controls. .. The reversal of MDR mediated by P-gp and the mechanism of cellular death was evaluated using the following monoclonal antibodies against: caspase-3 (sc-56053, Santa Cruz Biotechnology), ABCB1 (sc-55510, Santa Cruz Biotechnology), ABCG2 (sc-58224, Santa Cruz Biotechnology), horseradish peroxidase (HRP)-conjugated β-actin loading control monoclonal antibody (sc-47778, Santa Cruz Biotechnology), and HRP-conjugated antimouse IgG secondary antibody (ab6728, Abcam). .. Cells were collected from 6-well plates (5 × 10 4 cells/well), which were treated with or without different concentrations of compound 2 for 48 h. Later, cells were treated with lysis buffer containing 292 mM saccharose, 100 mM Tris (pH 7.6), 5 mM magnesium chloride (MgCl 2 ), 0.1% (w/v) sodium dodecyl sulfate (SDS), 1% (w/v) nonyl phenoxypolyethoxylethanol (NP40), 5 mM ethylenediamine tetraacetic acid (EDTA), and protease inhibitor (P9599, Sigma-Aldrich).

Transfection:

Article Title: Globo H ceramide confers chemoresistance and poor prognosis to advanced gallbladder cancer via A2AR/cAMP/PKA pathway
Article Snippet: .. For siRNA experiments, SNU308, TGBC24, and GBC01 cells were transfected with small interfering RNAs (siRNAs) targeting PRKACA (sc-36240, Santa Cruz Biotechnology, USA), A2AR (sc-39850, Santa Cruz Biotechnology, USA) or ABCG2 (sc-41151, Santa Cruz Biotechnology, USA) using Lipofectamine RNAiMAX (Thermo Fisher Scientific, MA, USA) according to the manufacturer's instructions. .. Briefly, cells were seeded into 6-well plates at a density of 1 × 10 5 cells/well, and transfection complexes were prepared by mixing siRNAs with Lipofectamine RNAiMAX in Opti-MEM medium (Thermo Fisher Scientific, MA, USA).

Article Title: Globo H ceramide confers chemoresistance and poor prognosis to advanced gallbladder cancer via A2AR/cAMP/PKA pathway.
Article Snippet: .. At the indicated time points, supernatants were collected to determine cAMP levels. siRNA Knock-down and cAMP Measurement For siRNA experiments, SNU308, TGBC24, and GBC01 cells were transfected with small interfering RNAs (siRNAs) targeting PRKACA (sc-36240, Santa Cruz Biotechnology, USA), A2AR (sc-39850, Santa Cruz Biotechnology, USA) or ABCG2 (sc-41151, Santa Cruz Biotechnology, USA) using Lipofectamine RNAiMAX (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s instructions. .. Briefly, cells were seeded into 6-well plates at a density of 1 × 105 cells/well, and transfection complexes were prepared by mixing siRNAs with Lipofectamine RNAiMAX in Opti-MEM medium (Thermo Fisher Scientific, MA, USA).

Incubation:

Article Title: Desmodium caudatum (Thunb.) DC. Extract Attenuates Hyperuricemia-Induced Renal Fibrosis via Modulating TGF-β1 Pathway and Uric Acid Transporters: Evidence from In Vitro and In Vivo Studies.
Article Snippet: Ethnopharmacological relevance: Desmodium caudatum (Thunb.). DC., a traditional Chinese medicinal herb, has been used to treat conditions such as rheumatic back pain, diarrhea, jaundice-related hepatitis, and abscesses; it also serves as an anthelmintic.. The extract of Desmodium caudatum (Thunb.)

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monoclonal mouse anti abcg2 antibody (Santa Cruz Biotechnology)

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1) Product Images from "Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays"

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